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Enzymatic and endpoint methods yield comparable adenosine deaminase activity in pleural fluid samples

Musharaf Tarajia, Emines Salas, Jacobus De Waard, Amador Goodridge.

Abstract

The biomarkers available for extrapulmonary tuberculosis diagnosis are limited. Adenosine deaminase (ADA; EC.3.5.4.4) has been employed extensively to diagnose pleural tuberculosis (PT). Similarly, ADA-2 isoenzyme activity also serves to increase the sensitivity and specificity of PT diagnosis.  However, the use of different methods to measure ADA and ADA-2 without standardization between laboratories could cause differences in ADA activity values, thus affecting PT diagnosis cut-off values.  In the present study, we aimed to depict the correlation between the Diazyme® ADA assay and the Giusti-Galanti method for measuring total ADA and ADA-2 isoenzyme in pleural fluid.

Musharaf Tarajia, Emines Salas, Jacobus De Waard, Amador Goodridge. Clinical Chemistry Laboratory Medicine, 2014 in press.