Tissue Engineering Part A 2019.0277

Role of region-specific brain decellularized extracellular matrix on in vitro neuronal maturation.

Diego Reginensi, Didio Ortiz, Andrea Pravia, Andrea Burillo, Félix Morales, Carly Morgan, Lindsay Jimenez, Kunjan R. Dave, Miguel A. Perez-Pinzon, Rolando A. Gittens.

Abstract:

ecent advancements in tissue engineering suggest that bioma-terials, such as decellularized extracellular matrix (ECM), could serve to potentiate the localization and efficacy of regenerative therapies in the central nervous system. Still, what factors and which mechanisms are required from these ECM-based biomateri-als to exert their effect are not entirely understood. In this study, we use the brain as a novel model to test the effects of particular biochemical and structural properties by evaluating, for the first time, three different sections of the brain (i.e., cortex, cerebel-lum, and remaining areas) side-by-side and their corresponding decellularized counterparts using mechanical (4-day) and chemi-cal (1-day) decellularization protocols. The three different brain subregions had considerably different initial conditions in terms of cell number and growth factor content, and some of these dif-ferences were maintained after decellularization. Decellularized ECM from both protocols was used as a substrate or as soluble factor, in both cases showing good cell attachment and growth capabilities. Interestingly, the 1-day protocol was capable of pro-moting greater differentiation than the 4-day protocol, probably due to its capacity to remove a similar amount of cell nuclei, while better conserving the biochemical and structural components of the cerebral ECM. Still, some limitations of this study include the need to evaluate the response in other biologically relevant cell types, as well as a more detailed characterization of the compo-nents in the decellularized ECM of the different brain subregions. In conclusion, our results show differences in neuronal matura-tion depending on the region of the brain used to produce the scaffolds. Complex organs such as the brain have subregions with very different initial cellular and biochemical conditions that should be considered for decellularization to minimize exposure to immunogenic components, while retaining bioactive factors conducive to regeneration.